lipid droplets (LDs) is the endoplasmic reticulum (ER) -derived lipid storage organelles is uniquely packed by phospholipid monolayers. LD membrane proteins are embedded into the topology monolayer monotopic hairpin and therefore tend to have different requirements for their biogenesis of people Insert as bitopic and polytopic protein to phospholipid bilayers. UBXD8 hairpin belonging to subfamily proteins that localize to the ER and LDS, and initially incorporated into the cytoplasm of the bilayer leaflets ER before Partition LD monolayer.
The molecular machinery responsible for protein hairpin insert into the membrane, however, is unknown. Here, we reported that newly synthesized UBXD8 is a post-translationally inserted into discrete ER subdomains with a mechanism that requires PEX19 cytosolic and membrane-integrated PEX3, until now exclusively proteins involved in peroxisome biogenesis.
Farnesylation of PEX19 Separating ER / LD and peroxisome targeting, expand the functions of this peroxin for ER-targeting pathway and suggested coordinated LDs and peroxisome biogenesis.
Human Peroxin Is PEX3 Integrated Co-translationally into the ER and ER in the budding vesicles Exit.
The old paradigm that all peroxisomal proteins post-translationally imported into the pre-existing peroxisomes has been challenged by the detection of peroxisomal membrane proteins (PMPs) in the endoplasmic reticulum (ER). In mammals, the entry mechanism ER and out of PMPs really unknown. We showed that the human PMP PEX3 insert co-translationally into the ER mammals through translocon Sec61.
Photocrosslinking and fluorescence spectroscopy studies showed that the N-terminal transmembrane segment (TMS) on the ribosome-bound PEX3 recognized by the signal recognition particle (SRP). Binding to SRP is a prerequisite to targeting of PEX3 containing ribosome nascent chain complex • (RNC) to translocon, where the integrated command lines nascent stages of the chain into the adjacent membrane protein Sec61α translocon and TRAM.
This insertion into the ER PEX3 physiologically relevant because PEX3 then exit ER through beginner vesicles in an ATP-dependent process. This research identified the initial steps in the human peroxisomal biogenesis by showing successive stages from the PMP through the mammalian ER.
Early-Acting Is PEX19 excessive Peroxin Encoding, farnesylated, and essential for viability in Arabidopsis thaliana.
Peroxisomes is a membrane-bound organelles that are essential for normal development in plants and animals. In mammalian and yeast, peroxin the (PEX) and PEX19 PEX3 protein facilitates the first step peroxisome membrane proteins (PMP) insertion and pre-peroxisome bud from the endoplasmic reticulum. PEX3 membrane proteins act as docking sites for PEX19, cytosolic chaperone for PMPs that give PMPs into the membrane of the endoplasmic reticulum or peroxisomal.
PEX19 is farnesylated in yeast and mammals, and we used immunoblotting with mutant prenilasi to show that PEX19 also fully farnesylated in wild-type Arabidopsis thaliana plants. We checked insertional alleles interfere with one of the two isoforms of Arabidopsis PEX19, PEX19A or PEX19B, and detected at the same level of protein PEX19 in pex19a-1 mutant and wild species; However, PEX19 protein is almost undetectable in pex19b-1 mutants. Despite the decline in the level of PEX19 in pex19b-1, both pex19a-1 and pex19b-1 single mutants shortage peroxisomal β-oxidation defect known and displayed normal levels and localization of peroxisomal matrix and membrane proteins.
 PEX12 siRNA |
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20-abx928274 |
Abbexa |
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PEX12 siRNA |
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20-abx928275 |
Abbexa |
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PEX12 siRNA |
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20-abx903946 |
Abbexa |
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 PEX12 Antibody |
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46123-100ul |
SAB |
100ul |
EUR 252 |
PEX12 Antibody |
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46123-50ul |
SAB |
50ul |
EUR 187 |
) Anti-PEX12 (2G6) |
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YF-MA14670 |
Abfrontier |
100 ug |
EUR 363 |
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Description: Mouse monoclonal to PEX12 |
 PEX12 Blocking Peptide |
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DF9739-BP |
Affbiotech |
1mg |
EUR 195 |
 PEX12 Polyclonal Antibody |
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ABP59879-003ml |
Abbkine |
0.03ml |
EUR 158 |
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Description: A polyclonal antibody for detection of PEX12 from Human, Mouse, Rat. This PEX12 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX12 protein at amino acid sequence of 180-260 |
PEX12 Polyclonal Antibody |
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ABP59879-01ml |
Abbkine |
0.1ml |
EUR 289 |
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Description: A polyclonal antibody for detection of PEX12 from Human, Mouse, Rat. This PEX12 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX12 protein at amino acid sequence of 180-260 |
PEX12 Polyclonal Antibody |
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ABP59879-02ml |
Abbkine |
0.2ml |
EUR 414 |
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Description: A polyclonal antibody for detection of PEX12 from Human, Mouse, Rat. This PEX12 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX12 protein at amino acid sequence of 180-260 |
 PEX12 Rabbit pAb |
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A16062-100ul |
Abclonal |
100 ul |
EUR 308 |
PEX12 Rabbit pAb |
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A16062-200ul |
Abclonal |
200 ul |
EUR 459 |
PEX12 Rabbit pAb |
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A16062-20ul |
Abclonal |
20 ul |
EUR 183 |
PEX12 Rabbit pAb |
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A16062-50ul |
Abclonal |
50 ul |
EUR 223 |
 Anti-PEX12 antibody |
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STJ191143 |
St John's Laboratory |
200 µl |
EUR 197 |
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Description: Unconjugated Rabbit polyclonal to PEX12 |
PEX12 Polyclonal Antibody |
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ES9985-100ul |
ELK Biotech |
100ul |
EUR 279 |
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Description: A Rabbit Polyclonal antibody against PEX12 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA |
PEX12 Polyclonal Antibody |
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ES9985-50ul |
ELK Biotech |
50ul |
EUR 207 |
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Description: A Rabbit Polyclonal antibody against PEX12 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA |
 PEX12 Conjugated Antibody |
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C46123 |
SAB |
100ul |
EUR 397 |
) PEX12 Recombinant Protein (Human) |
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RP082833 |
ABM |
100 ug |
Ask for price |
) PEX12 Recombinant Protein (Mouse) |
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RP161378 |
ABM |
100 ug |
Ask for price |
) PEX12 Recombinant Protein (Rat) |
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RP220079 |
ABM |
100 ug |
Ask for price |
 Rat PEX12 shRNA Plasmid |
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20-abx987350 |
Abbexa |
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 Mouse PEX12 shRNA Plasmid |
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20-abx979593 |
Abbexa |
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 Human PEX12 shRNA Plasmid |
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20-abx953465 |
Abbexa |
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) Polyclonal PEX12 Antibody (Center) |
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AMM08662G |
Leading Biology |
0.1ml |
EUR 528 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PEX12 (Center). This antibody is tested and proven to work in the following applications: |
 (pORF)) Pex12 ORF Vector (Rat) (pORF) |
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ORF073361 |
ABM |
1.0 ug DNA |
EUR 506 |
 (pORF)) PEX12 ORF Vector (Human) (pORF) |
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ORF027612 |
ABM |
1.0 ug DNA |
EUR 405 |
 (pORF)) Pex12 ORF Vector (Mouse) (pORF) |
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ORF053794 |
ABM |
1.0 ug DNA |
EUR 506 |
) Polyclonal PEX12 Antibody (C-Term) |
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AMM07097G |
Leading Biology |
0.1 mg |
EUR 484 |
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Description: A polyclonal antibody raised in Goat that recognizes and binds to Human PEX12 (C-Term). This antibody is tested and proven to work in the following applications: |
) Pex12 sgRNA CRISPR Lentivector set (Rat) |
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K7100901 |
ABM |
3 x 1.0 ug |
EUR 339 |
) Pex12 sgRNA CRISPR Lentivector set (Mouse) |
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K4677401 |
ABM |
3 x 1.0 ug |
EUR 339 |
) PEX12 sgRNA CRISPR Lentivector set (Human) |
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K1629501 |
ABM |
3 x 1.0 ug |
EUR 339 |
 Antibody) Peroxisome Assembly Protein 12 (PEX12) Antibody |
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abx431009-200ul |
Abbexa |
200 ul |
EUR 286 |
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Peroxisome Assembly Protein 12 (PEX12) Antibody |
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20-abx217718 |
Abbexa |
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Peroxisome Assembly Protein 12 (PEX12) Antibody |
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abx034437-400ul |
Abbexa |
400 ul |
EUR 523 |
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Peroxisome Assembly Protein 12 (PEX12) Antibody |
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abx034437-80l |
Abbexa |
80 µl |
EUR 286 |
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 PEX12 3'UTR Luciferase Stable Cell Line |
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TU017753 |
ABM |
1.0 ml |
EUR 1394 |
 Pex12 3'UTR Luciferase Stable Cell Line |
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TU116208 |
ABM |
1.0 ml |
Ask for price |
 Pex12 3'UTR GFP Stable Cell Line |
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TU166208 |
ABM |
1.0 ml |
Ask for price |
Pex12 3'UTR Luciferase Stable Cell Line |
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TU216093 |
ABM |
1.0 ml |
Ask for price |
Pex12 3'UTR GFP Stable Cell Line |
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TU266093 |
ABM |
1.0 ml |
Ask for price |
 PEX12 3'UTR GFP Stable Cell Line |
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TU067753 |
ABM |
1.0 ml |
EUR 1394 |
 (pPB-C-His)) PEX12 Protein Vector (Human) (pPB-C-His) |
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PV110446 |
ABM |
500 ng |
EUR 552 |
 (pPB-N-His)) PEX12 Protein Vector (Human) (pPB-N-His) |
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PV110447 |
ABM |
500 ng |
EUR 552 |
 (pPM-C-HA)) PEX12 Protein Vector (Human) (pPM-C-HA) |
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PV110448 |
ABM |
500 ng |
EUR 552 |
 (pPM-C-His)) PEX12 Protein Vector (Human) (pPM-C-His) |
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PV110449 |
ABM |
500 ng |
EUR 552 |
 (pPB-C-His)) PEX12 Protein Vector (Rat) (pPB-C-His) |
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PV293442 |
ABM |
500 ng |
EUR 603 |
 (pPB-N-His)) PEX12 Protein Vector (Rat) (pPB-N-His) |
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PV293443 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-HA)) PEX12 Protein Vector (Rat) (pPM-C-HA) |
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PV293444 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-His)) PEX12 Protein Vector (Rat) (pPM-C-His) |
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PV293445 |
ABM |
500 ng |
EUR 603 |
 (pPB-C-His)) PEX12 Protein Vector (Mouse) (pPB-C-His) |
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PV215174 |
ABM |
500 ng |
EUR 603 |
 (pPB-N-His)) PEX12 Protein Vector (Mouse) (pPB-N-His) |
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PV215175 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-HA)) PEX12 Protein Vector (Mouse) (pPM-C-HA) |
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PV215176 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-His)) PEX12 Protein Vector (Mouse) (pPM-C-His) |
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PV215177 |
ABM |
500 ng |
EUR 603 |
 (Target 1)) Pex12 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K7100902 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 2)) Pex12 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K7100903 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 3)) Pex12 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K7100904 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 1)) Pex12 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K4677402 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 2)) Pex12 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K4677403 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 3)) Pex12 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K4677404 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 1)) PEX12 sgRNA CRISPR Lentivector (Human) (Target 1) |
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K1629502 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 2)) PEX12 sgRNA CRISPR Lentivector (Human) (Target 2) |
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K1629503 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 3)) PEX12 sgRNA CRISPR Lentivector (Human) (Target 3) |
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K1629504 |
ABM |
1.0 ug DNA |
EUR 154 |
 Bovine Peroxisome assembly protein 12, PEX12 ELISA KIT |
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ELI-20906b |
Lifescience Market |
96 Tests |
EUR 928 |
 Human Peroxisome assembly protein 12, PEX12 ELISA KIT |
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ELI-20907h |
Lifescience Market |
96 Tests |
EUR 824 |
 Mouse Peroxisome assembly protein 12, Pex12 ELISA KIT |
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ELI-44896m |
Lifescience Market |
96 Tests |
EUR 865 |
 (CMV) (pLenti-GIII-CMV)) PEX12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV695353 |
ABM |
1.0 ug DNA |
EUR 682 |
 (UbC) (pLenti-GIII-UbC)) PEX12 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV695357 |
ABM |
1.0 ug DNA |
EUR 682 |
 (EF1a) (pLenti-GIII-EF1a)) PEX12 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV695358 |
ABM |
1.0 ug DNA |
EUR 682 |
) Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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K7100905 |
ABM |
3 x 1.0 ug |
EUR 376 |
) Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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K4677405 |
ABM |
3 x 1.0 ug |
EUR 376 |
) PEX12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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K1629505 |
ABM |
3 x 1.0 ug |
EUR 376 |
 (CMV) (pLenti-GIII-CMV-C-term-HA)) PEX12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV695354 |
ABM |
1.0 ug DNA |
EUR 682 |
 (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)) PEX12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV695355 |
ABM |
1.0 ug DNA |
EUR 740 |
 (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)) PEX12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV695356 |
ABM |
1.0 ug DNA |
EUR 740 |
 (Target 1)) Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1) |
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K7100906 |
ABM |
1.0 ug DNA |
EUR 167 |
 (Target 2)) Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2) |
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K7100907 |
ABM |
1.0 ug DNA |
EUR 167 |
 (Target 3)) Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3) |
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K7100908 |
ABM |
1.0 ug DNA |
EUR 167 |
 (Target 1)) Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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K4677406 |
ABM |
1.0 ug DNA |
EUR 167 |
 (Target 2)) Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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K4677407 |
ABM |
1.0 ug DNA |
EUR 167 |
 (Target 3)) Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
|
K4677408 |
ABM |
1.0 ug DNA |
EUR 167 |
 (Target 1)) PEX12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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K1629506 |
ABM |
1.0 ug DNA |
EUR 167 |
 (Target 2)) PEX12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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K1629507 |
ABM |
1.0 ug DNA |
EUR 167 |
 (Target 3)) PEX12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
|
K1629508 |
ABM |
1.0 ug DNA |
EUR 167 |
The pex19a-1 pex19b-1 double mutant is embryonic lethal, indicating the important role redundant coded to PEX19 during embryogenesis. Expressing YFP-tagged versions of both these deadly saved PEX19 isoforms, confirming that PEX19A and PEX19B overreacted in Arabidopsis. We observed that pex19b-1 enhanced the peroxisome related def
