Peroxin-dependent targeting of a lipid-droplet-destined membrane protein to ER subdomains.

lipid droplets (LDs) is the endoplasmic reticulum (ER) -derived lipid storage organelles is uniquely packed by phospholipid monolayers. LD membrane proteins are embedded into the topology monolayer monotopic hairpin and therefore tend to have different requirements for their biogenesis of people Insert as bitopic and polytopic protein to phospholipid bilayers. UBXD8 hairpin belonging to subfamily proteins that localize to the ER and LDS, and initially incorporated into the cytoplasm of the bilayer leaflets ER before Partition LD monolayer.

The molecular machinery responsible for protein hairpin insert into the membrane, however, is unknown. Here, we reported that newly synthesized UBXD8 is a post-translationally inserted into discrete ER subdomains with a mechanism that requires PEX19 cytosolic and membrane-integrated PEX3, until now exclusively proteins involved in peroxisome biogenesis.

Farnesylation of PEX19 Separating ER / LD and peroxisome targeting, expand the functions of this peroxin for ER-targeting pathway and suggested coordinated LDs and peroxisome biogenesis.

Human Peroxin Is PEX3 Integrated Co-translationally into the ER and ER in the budding vesicles Exit.

The old paradigm that all peroxisomal proteins post-translationally imported into the pre-existing peroxisomes has been challenged by the detection of peroxisomal membrane proteins (PMPs) in the endoplasmic reticulum (ER). In mammals, the entry mechanism ER and out of PMPs really unknown. We showed that the human PMP PEX3 insert co-translationally into the ER mammals through translocon Sec61.

Photocrosslinking and fluorescence spectroscopy studies showed that the N-terminal transmembrane segment (TMS) on the ribosome-bound PEX3 recognized by the signal recognition particle (SRP). Binding to SRP is a prerequisite to targeting of PEX3 containing ribosome nascent chain complex • (RNC) to translocon, where the integrated command lines nascent stages of the chain into the adjacent membrane protein Sec61α translocon and TRAM.

This insertion into the ER PEX3 physiologically relevant because PEX3 then exit ER through beginner vesicles in an ATP-dependent process. This research identified the initial steps in the human peroxisomal biogenesis by showing successive stages from the PMP through the mammalian ER.

Early-Acting Is PEX19 excessive Peroxin Encoding, farnesylated, and essential for viability in Arabidopsis thaliana.

Peroxisomes is a membrane-bound organelles that are essential for normal development in plants and animals. In mammalian and yeast, peroxin the (PEX) and PEX19 PEX3 protein facilitates the first step peroxisome membrane proteins (PMP) insertion and pre-peroxisome bud from the endoplasmic reticulum. PEX3 membrane proteins act as docking sites for PEX19, cytosolic chaperone for PMPs that give PMPs into the membrane of the endoplasmic reticulum or peroxisomal.

PEX19 is farnesylated in yeast and mammals, and we used immunoblotting with mutant prenilasi to show that PEX19 also fully farnesylated in wild-type Arabidopsis thaliana plants. We checked insertional alleles interfere with one of the two isoforms of Arabidopsis PEX19, PEX19A or PEX19B, and detected at the same level of protein PEX19 in pex19a-1 mutant and wild species; However, PEX19 protein is almost undetectable in pex19b-1 mutants. Despite the decline in the level of PEX19 in pex19b-1, both pex19a-1 and pex19b-1 single mutants shortage peroxisomal β-oxidation defect known and displayed normal levels and localization of peroxisomal matrix and membrane proteins.

PEX12 siRNA

20-abx928274
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PEX12 siRNA

20-abx928275
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PEX12 siRNA

20-abx903946
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PEX12 Antibody

46123-100ul 100ul
EUR 252

PEX12 Antibody

46123-50ul 50ul
EUR 187

Anti-PEX12 (2G6)

YF-MA14670 100 ug
EUR 363
Description: Mouse monoclonal to PEX12

PEX12 Blocking Peptide

DF9739-BP 1mg
EUR 195

PEX12 Polyclonal Antibody

ABP59879-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of PEX12 from Human, Mouse, Rat. This PEX12 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX12 protein at amino acid sequence of 180-260

PEX12 Polyclonal Antibody

ABP59879-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of PEX12 from Human, Mouse, Rat. This PEX12 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX12 protein at amino acid sequence of 180-260

PEX12 Polyclonal Antibody

ABP59879-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of PEX12 from Human, Mouse, Rat. This PEX12 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX12 protein at amino acid sequence of 180-260

PEX12 Rabbit pAb

A16062-100ul 100 ul
EUR 308

PEX12 Rabbit pAb

A16062-200ul 200 ul
EUR 459

PEX12 Rabbit pAb

A16062-20ul 20 ul
EUR 183

PEX12 Rabbit pAb

A16062-50ul 50 ul
EUR 223

Anti-PEX12 antibody

STJ118515 100 µl
EUR 277

Anti-PEX12 antibody

STJ191143 200 µl
EUR 197
Description: Unconjugated Rabbit polyclonal to PEX12

PEX12 Polyclonal Antibody

ES9985-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against PEX12 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

PEX12 Polyclonal Antibody

ES9985-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against PEX12 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti-PEX12 antibody

STJ72257 100 µg
EUR 260

PEX12 Conjugated Antibody

C46123 100ul
EUR 397

PEX12 Recombinant Protein (Human)

RP082833 100 ug Ask for price

PEX12 Recombinant Protein (Mouse)

RP161378 100 ug Ask for price

PEX12 Recombinant Protein (Rat)

RP220079 100 ug Ask for price

Rat PEX12 shRNA Plasmid

20-abx987350
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse PEX12 shRNA Plasmid

20-abx979593
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human PEX12 shRNA Plasmid

20-abx953465
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Polyclonal PEX12 Antibody (Center)

AMM08662G 0.1ml
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PEX12 (Center). This antibody is tested and proven to work in the following applications:

Pex12 ORF Vector (Rat) (pORF)

ORF073361 1.0 ug DNA
EUR 506

PEX12 ORF Vector (Human) (pORF)

ORF027612 1.0 ug DNA
EUR 405

Pex12 ORF Vector (Mouse) (pORF)

ORF053794 1.0 ug DNA
EUR 506

Polyclonal PEX12 Antibody (C-Term)

AMM07097G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human PEX12 (C-Term). This antibody is tested and proven to work in the following applications:

Pex12 sgRNA CRISPR Lentivector set (Rat)

K7100901 3 x 1.0 ug
EUR 339

Pex12 sgRNA CRISPR Lentivector set (Mouse)

K4677401 3 x 1.0 ug
EUR 339

PEX12 sgRNA CRISPR Lentivector set (Human)

K1629501 3 x 1.0 ug
EUR 339

Peroxisome Assembly Protein 12 (PEX12) Antibody

abx431009-200ul 200 ul
EUR 286

Peroxisome Assembly Protein 12 (PEX12) Antibody

20-abx217718
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

Peroxisome Assembly Protein 12 (PEX12) Antibody

abx034437-400ul 400 ul
EUR 523

Peroxisome Assembly Protein 12 (PEX12) Antibody

abx034437-80l 80 µl
EUR 286

PEX12 3'UTR Luciferase Stable Cell Line

TU017753 1.0 ml
EUR 1394

Pex12 3'UTR Luciferase Stable Cell Line

TU116208 1.0 ml Ask for price

Pex12 3'UTR GFP Stable Cell Line

TU166208 1.0 ml Ask for price

Pex12 3'UTR Luciferase Stable Cell Line

TU216093 1.0 ml Ask for price

Pex12 3'UTR GFP Stable Cell Line

TU266093 1.0 ml Ask for price

PEX12 3'UTR GFP Stable Cell Line

TU067753 1.0 ml
EUR 1394

PEX12 Protein Vector (Human) (pPB-C-His)

PV110446 500 ng
EUR 552

PEX12 Protein Vector (Human) (pPB-N-His)

PV110447 500 ng
EUR 552

PEX12 Protein Vector (Human) (pPM-C-HA)

PV110448 500 ng
EUR 552

PEX12 Protein Vector (Human) (pPM-C-His)

PV110449 500 ng
EUR 552

PEX12 Protein Vector (Rat) (pPB-C-His)

PV293442 500 ng
EUR 603

PEX12 Protein Vector (Rat) (pPB-N-His)

PV293443 500 ng
EUR 603

PEX12 Protein Vector (Rat) (pPM-C-HA)

PV293444 500 ng
EUR 603

PEX12 Protein Vector (Rat) (pPM-C-His)

PV293445 500 ng
EUR 603

PEX12 Protein Vector (Mouse) (pPB-C-His)

PV215174 500 ng
EUR 603

PEX12 Protein Vector (Mouse) (pPB-N-His)

PV215175 500 ng
EUR 603

PEX12 Protein Vector (Mouse) (pPM-C-HA)

PV215176 500 ng
EUR 603

PEX12 Protein Vector (Mouse) (pPM-C-His)

PV215177 500 ng
EUR 603

Pex12 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7100902 1.0 ug DNA
EUR 154

Pex12 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7100903 1.0 ug DNA
EUR 154

Pex12 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7100904 1.0 ug DNA
EUR 154

Pex12 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4677402 1.0 ug DNA
EUR 154

Pex12 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4677403 1.0 ug DNA
EUR 154

Pex12 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4677404 1.0 ug DNA
EUR 154

PEX12 sgRNA CRISPR Lentivector (Human) (Target 1)

K1629502 1.0 ug DNA
EUR 154

PEX12 sgRNA CRISPR Lentivector (Human) (Target 2)

K1629503 1.0 ug DNA
EUR 154

PEX12 sgRNA CRISPR Lentivector (Human) (Target 3)

K1629504 1.0 ug DNA
EUR 154

Bovine Peroxisome assembly protein 12, PEX12 ELISA KIT

ELI-20906b 96 Tests
EUR 928

Human Peroxisome assembly protein 12, PEX12 ELISA KIT

ELI-20907h 96 Tests
EUR 824

Mouse Peroxisome assembly protein 12, Pex12 ELISA KIT

ELI-44896m 96 Tests
EUR 865

PEX12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV695353 1.0 ug DNA
EUR 682

PEX12 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV695357 1.0 ug DNA
EUR 682

PEX12 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV695358 1.0 ug DNA
EUR 682

Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7100905 3 x 1.0 ug
EUR 376

Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4677405 3 x 1.0 ug
EUR 376

PEX12 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1629505 3 x 1.0 ug
EUR 376

PEX12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV695354 1.0 ug DNA
EUR 682

PEX12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV695355 1.0 ug DNA
EUR 740

PEX12 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV695356 1.0 ug DNA
EUR 740

Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7100906 1.0 ug DNA
EUR 167

Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7100907 1.0 ug DNA
EUR 167

Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7100908 1.0 ug DNA
EUR 167

Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4677406 1.0 ug DNA
EUR 167

Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4677407 1.0 ug DNA
EUR 167

Pex12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4677408 1.0 ug DNA
EUR 167

PEX12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1629506 1.0 ug DNA
EUR 167

PEX12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1629507 1.0 ug DNA
EUR 167

PEX12 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1629508 1.0 ug DNA
EUR 167

The pex19a-1 pex19b-1 double mutant is embryonic lethal, indicating the important role redundant coded to PEX19 during embryogenesis. Expressing YFP-tagged versions of both these deadly saved PEX19 isoforms, confirming that PEX19A and PEX19B overreacted in Arabidopsis. We observed that pex19b-1 enhanced the peroxisome related def

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