Colletotrichum orbiculare FAM1 Encodes a Novel Woronin Body-Associated Pex22 Peroxin Required for Appressorium-Mediated Plant Infection.

Colletotrichum orbiculare FAM1 Encodes a Novel Woronin Body-Associated Pex22 Peroxin Required for Appressorium-Mediated Plant Infection.

Cucumber anthracnose fungus Colletotrichum orbiculare special form cells called appresoria to penetrate the host. We identified a gene, FAM1, peroxin encoding novel protein that is essential for peroxisome biogenesis and associates with Woronin body (WBS), dense-core vesicles are only found in filamentous ascomycete fungus that serves to maintain cellular integrity.

fam1 disrupted mutant can not grow on a medium containing oleic acid as the sole carbon source and pathogenic, became disabled in both melanisasi appresorium and penetration of the host. fluorescent proteins carry peroxisomal targeting signals (PTSs) are not imported into peroxisomes fam1 mutants, suggesting that FAM1 is new peroxisomal biogenesis genes (peroxin).

FAM1 showed no significant homology to any Saccharomyces cerevisiae peroxins but resemble filamentous ascomycete preserved special Pex22-like proteins containing predicted Pex4-binding sites and potentially involved in the recycling of receptors PTS of peroxisomes to the cytosol. C. orbiculare FAM1 complement defective peroxisomal matrix protein import of S. cerevisiae mutants pex22. Confocal microscopy of Fam1-GFP (green fluorescent protein) fusion proteins and immunoelectron microscopy with the antibody anti-Fam1 show that Fam1 WBS translated into newborn starter of peroxisomes and mature WBS.

Fam1 WBS Association confirmed by colocalization with WB CoHex1 matrix protein (C. orbiculare Hex1) and WB CoWsc membrane proteins (C. orbiculare WSC) and by subcellular fractionation and Western blotting with antibodies to Fam1 and CoHex1. In cohex1 WB-deficient mutants, Fam1 transferred to the membrane of the peroxisome. Our results indicate that the related WB peroxin Fam1 is required for pathogenesis and increase the likelihood that localized receptor recycling occurs at WBS.


OBJECTIVE
orbiculare Colletotrichum is a fungus that causes damage Cucurbitaceae plant diseases. In this paper, we characterize new gene peroxisome biogenesis of these pathogens is called FAM1. Although there are no genes with significant homology present in Saccharomyces cerevisiae, FAM1 contain binding sites predicted Pex4 typical Pex22 protein, which functions in receptor recycling PTS of peroxisomes to the cytosol.

We show that the complement FAM1 defect in peroxisomal matrix protein import mutant S. cerevisiae mutants fam1 pex22 and that really defective in peroxisome function, metabolism of fatty acids, and pathogenicity. Remarkably, we found that this novel peroxin specifically localized to the membrane of the body running Woronin, tiny organelles that are unique to the peroxisomes derived ascomycete fungus filaments that function in the septum pores insert. Our findings indicate that this fungus has been co-opted body for recycling Woronin local receptors for matrix protein import.

Colletotrichum orbiculare FAM1 Encodes a Novel Woronin Body-Associated Pex22 Peroxin Required for Appressorium-Mediated Plant Infection.
Colletotrichum orbiculare FAM1 Encodes a Novel Woronin Body-Associated Pex22 Peroxin Required for Appressorium-Mediated Plant Infection.

Ancestral role in peroxisome assembly maintained by Pex11 peroxin division in yeast Yarrowia lipolytica.

The peroxin Pex11 have a recognized role in peroxisome division. Pex11p remodels and extends peroxisomal membrane prior to recruitment Dynamin-related GTPases that act in cutting the membrane to divide the peroxisomes. We conducted a comprehensive survey of comparative genomics to understand the meaning of the evolution of a family of proteins Pex11 in yeast and other eukaryotes.

anti-PEX19

YF-PA14237 50 ul
EUR 363
Description: Mouse polyclonal to PEX19

anti-PEX19

YF-PA14238 50 ug
EUR 363
Description: Mouse polyclonal to PEX19

anti-PEX19

YF-PA14239 100 ug
EUR 403
Description: Rabbit polyclonal to PEX19

PEX19 antibody

70R-19221 50 ul
EUR 435
Description: Rabbit polyclonal PEX19 antibody

PEX19 antibody

70R-10334 50 ug
EUR 467
Description: Affinity purified rabbit polyclonal PEX19 antibody

PEX19 antibody

70R-13383 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal PEX19 antibody

PEX19 Antibody

DF4281 200ul
EUR 304
Description: PEX19 Antibody detects endogenous levels of total PEX19.

PEX19 Antibody

DF7361 200ul
EUR 304
Description: PEX19 Antibody detects endogenous levels of total PEX19.

PEX19 Antibody

1-CSB-PA040121
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PEX19. Recognizes PEX19 from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/5000

PEX19 Antibody

1-CSB-PA017802DSR2HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against PEX19. Recognizes PEX19 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

PEX19 Antibody

1-CSB-PA017802GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against PEX19. Recognizes PEX19 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

pOTB7-PEX19

PVT18412 2 ug
EUR 231

PEX19 siRNA

20-abx928282
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  • 15 nmol
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PEX19 siRNA

20-abx928283
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PEX19 antibody

22022-100ul 100ul
EUR 390

PEX19 Antibody

32874-100ul 100ul
EUR 252

Anti-PEX19 (2E4)

YF-MA15070 100 ug
EUR 363
Description: Mouse monoclonal to PEX19

PEX19 Blocking Peptide

33R-10300 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of PEX19 antibody, catalog no. 70R-10334

PEX19 Blocking Peptide

DF4281-BP 1mg
EUR 195

PEX19 Blocking Peptide

DF7361-BP 1mg
EUR 195

Anti-PEX19 antibody

PAab06329 100 ug
EUR 412

PEX19 cloning plasmid

CSB-CL017802HU-10ug 10ug
EUR 233
Description: A cloning plasmid for the PEX19 gene.

Human PEX19 Protein

20-abx650904
  • EUR 411.00
  • EUR 217.00
  • EUR 1052.00
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  • 100 ug
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Mouse PEX19 Protein

20-abx650905
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  • EUR 244.00
  • EUR 1553.00
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PEX19 Blocking Peptide

20-abx061308
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PEX19 Rabbit pAb

A5476-100ul 100 ul
EUR 308

PEX19 Rabbit pAb

A5476-200ul 200 ul
EUR 459

PEX19 Rabbit pAb

A5476-20ul 20 ul
EUR 183

PEX19 Rabbit pAb

A5476-50ul 50 ul
EUR 223

Human PEX19 Antibody

32561-05111 150 ug
EUR 261

Anti-PEX19 antibody

STJ27429 100 µl
EUR 277
Description: This gene is necessary for early peroxisomal biogenesis. It acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. The peroxisome biogenesis disorders (PBDs) are a group of genetically heterogeneous autosomal recessive, lethal diseases characterized by multiple defects in peroxisome function. These disorders have at least 14 complementation groups, with more than one phenotype being observed for some complementation groups. Although the clinical features of PBD patients vary, cells from all PBD patients exhibit a defect in the import of one or more classes of peroxisomal matrix proteins into the organelle. Defects in this gene are a cause of Zellweger syndrome (ZWS), as well as peroxisome biogenesis disorder complementation group 14 (PBD-CG14), which is also known as PBD-CGJ. Alternative splicing results in multiple transcript variants.

anti- PEX19 antibody

FNab06329 100µg
EUR 585
Description: Antibody raised against PEX19

PEX19 Conjugated Antibody

C32874 100ul
EUR 397

PEX19 protein (His tag)

80R-1957 100 ug
EUR 322
Description: Recombinant human PEX19 protein

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EF001691 96 Tests
EUR 689

PEX19 Recombinant Protein (Human)

RP023137 100 ug Ask for price

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RP161390 100 ug Ask for price

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RP161393 100 ug Ask for price

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RP220091 100 ug Ask for price

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RP220094 100 ug Ask for price

Human PEX19 shRNA Plasmid

20-abx953936
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Pex19 ORF Vector (Rat) (pORF)

ORF073365 1.0 ug DNA
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Pex19 ORF Vector (Rat) (pORF)

ORF073366 1.0 ug DNA
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PEX19 ORF Vector (Human) (pORF)

ORF007713 1.0 ug DNA
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ORF053798 1.0 ug DNA
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Pex19 ORF Vector (Mouse) (pORF)

ORF053799 1.0 ug DNA
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Anti-PEX19/Peroxin 19 Antibody

A03186 100ul
EUR 397
Description: Rabbit Polyclonal PEX19/Peroxin 19 Antibody. Validated in WB and tested in Human.

Human PEX19 Antibody (Biotin Conjugate)

32561-05121 150 ug
EUR 369

Recombinant Peroxisomal Biogenesis Factor 19 (PEX19)

4-RPE733Hu01
  • EUR 279.20
  • EUR 178.00
  • EUR 772.00
  • EUR 324.00
  • EUR 548.00
  • EUR 250.00
  • EUR 1780.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Peroxisomal Biogenesis Factor 19 expressed in: E.coli

Recombinant Peroxisomal Biogenesis Factor 19 (PEX19)

4-RPE733Mu01
  • EUR 377.76
  • EUR 204.00
  • EUR 1141.60
  • EUR 447.20
  • EUR 794.40
  • EUR 316.00
  • EUR 2704.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Peroxisomal Biogenesis Factor 19 expressed in: E.coli

Pex19 sgRNA CRISPR Lentivector set (Rat)

K6286401 3 x 1.0 ug
EUR 339

Pex19 sgRNA CRISPR Lentivector set (Mouse)

K3114001 3 x 1.0 ug
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K1629901 3 x 1.0 ug
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Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

20-abx114407
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  • 150 ul
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Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

20-abx320664
  • EUR 439.00
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  • 100 ul
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Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

abx236329-100ug 100 ug
EUR 551

Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

20-abx249459
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

20-abx324739
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  • 100 ug
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Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

20-abx004196
  • EUR 411.00
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  • 100 ul
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Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

20-abx133726
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  • EUR 439.00
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Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

abx146374-100ug 100 ug
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Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

abx028024-400ul 400 ul
EUR 523

Peroxisomal Biogenesis Factor 19 (PEX19) Antibody

abx028024-80l 80 µl
EUR 286

Human PEX19 AssayLite Antibody (FITC Conjugate)

32561-05141 150 ug
EUR 428

Human PEX19 AssayLite Antibody (RPE Conjugate)

32561-05151 150 ug
EUR 428

Human PEX19 AssayLite Antibody (APC Conjugate)

32561-05161 150 ug
EUR 428

Human PEX19 AssayLite Antibody (PerCP Conjugate)

32561-05171 150 ug
EUR 471

PEX19 3'UTR Luciferase Stable Cell Line

TU017757 1.0 ml
EUR 1521

Pex19 3'UTR Luciferase Stable Cell Line

TU116212 1.0 ml Ask for price

Pex19 3'UTR GFP Stable Cell Line

TU166212 1.0 ml Ask for price

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TU216097 1.0 ml Ask for price

Pex19 3'UTR GFP Stable Cell Line

TU266097 1.0 ml Ask for price

PEX19 3'UTR GFP Stable Cell Line

TU067757 1.0 ml
EUR 1521

PEX19 Protein Vector (Rat) (pPB-C-His)

PV293458 500 ng
EUR 603

PEX19 Protein Vector (Rat) (pPB-N-His)

PV293459 500 ng
EUR 603

PEX19 Protein Vector (Rat) (pPM-C-HA)

PV293460 500 ng
EUR 603

PEX19 Protein Vector (Rat) (pPM-C-His)

PV293461 500 ng
EUR 603

PEX19 Protein Vector (Rat) (pPB-C-His)

PV293462 500 ng
EUR 603

PEX19 Protein Vector (Rat) (pPB-N-His)

PV293463 500 ng
EUR 603

PEX19 Protein Vector (Rat) (pPM-C-HA)

PV293464 500 ng
EUR 603

PEX19 Protein Vector (Rat) (pPM-C-His)

PV293465 500 ng
EUR 603

PEX19 Protein Vector (Mouse) (pPB-C-His)

PV215190 500 ng
EUR 603

PEX19 Protein Vector (Mouse) (pPB-N-His)

PV215191 500 ng
EUR 603

PEX19 Protein Vector (Mouse) (pPM-C-HA)

PV215192 500 ng
EUR 603

PEX19 Protein Vector (Mouse) (pPM-C-His)

PV215193 500 ng
EUR 603

PEX19 Protein Vector (Mouse) (pPB-C-His)

PV215194 500 ng
EUR 603

PEX19 Protein Vector (Mouse) (pPB-N-His)

PV215195 500 ng
EUR 603

PEX19 Protein Vector (Mouse) (pPM-C-HA)

PV215196 500 ng
EUR 603

PEX19 Protein Vector (Mouse) (pPM-C-His)

PV215197 500 ng
EUR 603

PEX19 Protein Vector (Human) (pPB-C-His)

PV030849 500 ng
EUR 329

PEX19 Protein Vector (Human) (pPB-N-His)

PV030850 500 ng
EUR 329

PEX19 Protein Vector (Human) (pPM-C-HA)

PV030851 500 ng
EUR 329

PEX19 Protein Vector (Human) (pPM-C-His)

PV030852 500 ng
EUR 329

Pex19 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6286402 1.0 ug DNA
EUR 154

Pex19 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6286403 1.0 ug DNA
EUR 154

Pex19 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6286404 1.0 ug DNA
EUR 154

Pex19 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3114002 1.0 ug DNA
EUR 154

Pex19 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3114003 1.0 ug DNA
EUR 154

Pex19 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3114004 1.0 ug DNA
EUR 154

PEX19 sgRNA CRISPR Lentivector (Human) (Target 1)

K1629902 1.0 ug DNA
EUR 154

PEX19 sgRNA CRISPR Lentivector (Human) (Target 2)

K1629903 1.0 ug DNA
EUR 154

PEX19 sgRNA CRISPR Lentivector (Human) (Target 3)

K1629904 1.0 ug DNA
EUR 154

Human Peroxisomal biogenesis factor 19, PEX19 ELISA KIT

ELI-21564h 96 Tests
EUR 824

Mouse Peroxisomal biogenesis factor 19, Pex19 ELISA KIT

ELI-37435m 96 Tests
EUR 865

Bovine Peroxisomal biogenesis factor 19, PEX19 ELISA KIT

ELI-37739b 96 Tests
EUR 928

PEX19 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV659233 1.0 ug DNA
EUR 514

PEX19 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV659237 1.0 ug DNA
EUR 514

PEX19 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV659238 1.0 ug DNA
EUR 514

PEX19 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV659299 1.0 ug DNA
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PEX19 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV659303 1.0 ug DNA
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PEX19 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV659304 1.0 ug DNA
EUR 514

Human Peroxisomal Biogenesis Factor 19 (PEX19) ELISA Kit

abx382162-96tests 96 tests
EUR 911

PEX19 Peroxisomal Biogenesis Factor 19 Human Recombinant Protein

PROTP40855 Regular: 20ug
EUR 317
Description: PEX19 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 316 amino acids (1-296) and having a molecular mass of 34.6 kDa.;The PEX19 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Recombinant Saccharomyces Cerevisiae PEX19 Protein (aa 1-338) [His]

VAng-Wyb5280-1mg 1 mg
EUR 4553
Description: Saccharomyces Cerevisiae (strain YJM789) (Bakers yeast) Peroxisomal Biogenesis Factor 19, recombinant protein.

Pex19 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6286405 3 x 1.0 ug
EUR 376

Pex19 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3114005 3 x 1.0 ug
EUR 376

PEX19 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1629905 3 x 1.0 ug
EUR 376

PEX19 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV659234 1.0 ug DNA
EUR 514

PEX19 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV659235 1.0 ug DNA
EUR 572

PEX19 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV659236 1.0 ug DNA
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PEX19 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV659300 1.0 ug DNA
EUR 514

PEX19 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV659301 1.0 ug DNA
EUR 572

PEX19 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV659302 1.0 ug DNA
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Pex19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6286406 1.0 ug DNA
EUR 167

Pex19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6286407 1.0 ug DNA
EUR 167

Pex19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6286408 1.0 ug DNA
EUR 167

Pex19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3114006 1.0 ug DNA
EUR 167

Pex19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3114007 1.0 ug DNA
EUR 167

Pex19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3114008 1.0 ug DNA
EUR 167

PEX19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1629906 1.0 ug DNA
EUR 167

PEX19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1629907 1.0 ug DNA
EUR 167

PEX19 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1629908 1.0 ug DNA
EUR 167

Pex11p highly conserved and ancestors, and has undergone a lot of duplication of the specific line, while the other Pex11 protein family members are certain fungal innovation.

Functional characterization of in-silico-predicted Pex11 family member proteins of the yeast Yarrowia lipolytica, namely Pex11p, Pex11Cp and Pex11 / 25p, suggesting that Pex11Cp and Pex11 / 25p have a role in the regulation of peroxisome size and number of family members Pex11 protein characteristics. Unexpectedly, deletion PEX11 in Y. lipolytica produce cells that lacked per identified morphological

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