Identification and functional characterization of Trypanosoma brucei peroxin 16.

Protozoan parasites of the family Trypanosomatidae infect humans as well as livestock such devastating disease causing sleeping sickness, Chagas disease and Leishmaniasis. This parasite glycolytic enzymes in the organelle through a unique, glycosomes.

Glycosomes is a subclass of peroxisomes and they are essential to the survival of the parasite. Therefore, interference glycosome biogenesis is an attractive drug target for this Neglected Tropical Diseases (NTDs). Peroxin 16 (PEX16) plays an important role in peroxisomal membrane protein targeting and de novo biogenesis of the peroxisomes from the endoplasmic reticulum (ER).

We identify trypanosomal PEX16 based on characteristics of a particular sequence and indicates that it is an integral membrane protein glycosomal of procyclic and bloodstream trypanosomes form. RNAi-mediated knockdown partial PEX16 Trypanosoma brucei bloodstream form trypanosomes causing severe ATP depletion, disability motility and cell death. Microscopic and biochemical analyzes revealed a drastic reduction glycosome and mislocalization amount of enzyme matrix glycosomal to the cytosol. Glycosomes localization remaining asymmetry observed with severe depletion in the posterior part.

The results showed that PEX16 trypanosomal very important for the biogenesis glycosome and thus, providing a potential drug target for sleeping sickness and related diseases.

Peroxin Pex14p are key components for autophagic degradation of mammalian peroxisomes coordinated by directly binding LC3-II.

Pexophagy can be experimentally induced in mammalian cells by removing serum culture. Pex14p, an important membrane protein peroxisomal for imported protein matrix in docking receptor soluble Pex5p, is involved in the degradation of autophagic mammals from peroxisomes and interact with forms lipidated of LC3, called LC3-II, is an important factor for the formation of autophagosome, under conditions of starvation in cell CHO-K1. However, the molecular mechanisms underlying the interaction Pex14p-LC3-II is still unknown.

To verify whether Pex14p directly bind LC3-II, we reconstituted in vitro system of conjugation for the synthesis of LC3-II. We show here that Pex14p directly interact with LC3-II through the transmembrane domain of Pex14p. Pex5p competitively inhibits this interaction, implying that Pex14p preferential binding to Pex5p under rich conditions nutrisi-. In addition, mutants defective in the import Pex5p PTS1-protein loses its affinity for Pex14p under conditions of malnutrition, so it might explain why Pex14p preferentially interact with LC3-II under starvation conditions in vivo.

PEX14 antibody
70R-19219	Fitzgerald	50 ul	EUR 435
Description: Rabbit polyclonal PEX14 antibody

PEX14 Antibody
DF4284	Affbiotech	200ul	EUR 304
Description: PEX14 Antibody detects endogenous levels of total PEX14.

PEX14 Antibody
1-CSB-PA017800ESR1HU	Cusabio	EUR 222.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

PEX14 Antibody
1-CSB-PA017800ESR2HU	Cusabio	EUR 222.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

PEX14 Antibody
1-CSB-PA017800GA01HU	Cusabio	EUR 597.00 EUR 333.00	150ul 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IF

PEX14 Antibody
1-CSB-PA867033LA01DOA	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Arabidopsis thaliana. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

PEX14 Antibody
1-CSB-PA003741	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

PEX14 Antibody
CSB-PA775947-	Cusabio		EUR 335
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

PEX14 Antibody
CSB-PA775947-100ul	Cusabio	100ul	EUR 316
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

PEX14 siRNA
20-abx928278	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

PEX14 siRNA
20-abx928279	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

PEX14 Antibody
34892-100ul	SAB	100ul	EUR 252

PEX14 Antibody
34892-50ul	SAB	50ul	EUR 187

PEX14 siRNA
20-abx903947	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

Human Peroxisomal membrane protein PEX14, PEX14 ELISA KIT
ELI-35662h	Lifescience Market	96 Tests	EUR 824

Mouse Peroxisomal membrane protein PEX14, Pex14 ELISA KIT
ELI-37738m	Lifescience Market	96 Tests	EUR 865

Anti-PEX14 (1G12)
YF-MA14671	Abfrontier	100 ug	EUR 363
Description: Mouse monoclonal to PEX14

PEX14 Blocking Peptide
DF4284-BP	Affbiotech	1mg	EUR 195

Anti-PEX14 antibody
PAab06327	Lifescience Market	100 ug	EUR 355

PEX14 cloning plasmid
CSB-CL017800HU-10ug	Cusabio	10ug	EUR 233
Description: A cloning plasmid for the PEX14 gene.

PEX14 Polyclonal Antibody
ES8932-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against PEX14 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

PEX14 Polyclonal Antibody
ES8932-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against PEX14 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

PEX14 Blocking Peptide
20-abx063068	Abbexa	EUR 272.00 EUR 411.00	1 mg 5 mg

Anti-PEX14 Antibody
A03327	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal PEX14 Antibody. Validated in IHC, WB and tested in Human, Mouse.

PEX14 Polyclonal Antibody
ABP59881-003ml	Abbkine	0.03ml	EUR 158
Description: A polyclonal antibody for detection of PEX14 from Human, Mouse, Rat. This PEX14 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX14 protein

PEX14 Polyclonal Antibody
ABP59881-01ml	Abbkine	0.1ml	EUR 289
Description: A polyclonal antibody for detection of PEX14 from Human, Mouse, Rat. This PEX14 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX14 protein

PEX14 Polyclonal Antibody
ABP59881-02ml	Abbkine	0.2ml	EUR 414
Description: A polyclonal antibody for detection of PEX14 from Human, Mouse, Rat. This PEX14 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX14 protein

Anti-PEX14 antibody
STJ29475	St John's Laboratory	100 µl	EUR 277
Description: This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.

Anti-PEX14 antibody
STJ99650	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to PEX14.

PEX14 Rabbit pAb
A7336-100ul	Abclonal	100 ul	EUR 308

PEX14 Rabbit pAb
A7336-200ul	Abclonal	200 ul	EUR 459

PEX14 Rabbit pAb
A7336-20ul	Abclonal	20 ul	EUR 183

PEX14 Rabbit pAb
A7336-50ul	Abclonal	50 ul	EUR 223

PEX14 Conjugated Antibody
C34892	SAB	100ul	EUR 397

anti- PEX14 antibody
FNab06327	FN Test	100µg	EUR 505.25
Description: Antibody raised against PEX14

PEX14 ELISA KIT|Human
EF001689	Lifescience Market	96 Tests	EUR 689

PEX14 Antibody, HRP conjugated
1-CSB-PA867033LB01DOA	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Arabidopsis thaliana. This antibody is HRP conjugated. Tested in the following application: ELISA

PEX14 Antibody, FITC conjugated
1-CSB-PA867033LC01DOA	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Arabidopsis thaliana. This antibody is FITC conjugated. Tested in the following application: ELISA

PEX14 Antibody, Biotin conjugated
1-CSB-PA867033LD01DOA	Cusabio	EUR 317.00 EUR 335.00	100ul 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Arabidopsis thaliana. This antibody is Biotin conjugated. Tested in the following application: ELISA

PEX14 Recombinant Protein (Human)
RP023131	ABM	100 ug	Ask for price

PEX14 Recombinant Protein (Mouse)
RP161384	ABM	100 ug	Ask for price

PEX14 Recombinant Protein (Rat)
RP220085	ABM	100 ug	Ask for price

Mouse PEX14 shRNA Plasmid
20-abx974669	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Rat PEX14 shRNA Plasmid
20-abx986246	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Human PEX14 shRNA Plasmid
20-abx953467	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Polyclonal PEX14 Antibody (Center)
AMM07100G	Leading Biology	0.1ml	EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PEX14 (Center). This antibody is tested and proven to work in the following applications:

Pex14 ORF Vector (Rat) (pORF)
ORF073363	ABM	1.0 ug DNA	EUR 506

PEX14 ORF Vector (Human) (pORF)
ORF007711	ABM	1.0 ug DNA	EUR 95

Pex14 ORF Vector (Mouse) (pORF)
ORF053796	ABM	1.0 ug DNA	EUR 506

Pex14 sgRNA CRISPR Lentivector set (Mouse)
K4712301	ABM	3 x 1.0 ug	EUR 339

Pex14 sgRNA CRISPR Lentivector set (Rat)
K7067601	ABM	3 x 1.0 ug	EUR 339

PEX14 sgRNA CRISPR Lentivector set (Human)
K1629701	ABM	3 x 1.0 ug	EUR 339

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
20-abx114405	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
abx034246-400ul	Abbexa	400 ul	EUR 523

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
abx034246-80l	Abbexa	80 µl	EUR 286

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
abx036238-100ug	Abbexa	100 ug	EUR 391

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
20-abx014702	Abbexa	EUR 314.00 EUR 98.00 EUR 398.00 EUR 495.00 EUR 70.00	100 ug 10 ug 200 ug 300 µg 5 ug

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
20-abx006919	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
20-abx008877	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
abx026888-400ul	Abbexa	400 ul	EUR 523

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
abx026888-80l	Abbexa	80 µl	EUR 286

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
20-abx320904	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
20-abx320905	Abbexa	EUR 439.00 EUR 328.00	100 ul 50 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
abx236327-100ug	Abbexa	100 ug	EUR 481

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
abx331597-100ul	Abbexa	100 ul	EUR 425

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody
20-abx328657	Abbexa	EUR 314.00 EUR 244.00	100 ug 50 ug

PEX14 3'UTR Luciferase Stable Cell Line
TU017755	ABM	1.0 ml	EUR 2333

Pex14 3'UTR Luciferase Stable Cell Line
TU116210	ABM	1.0 ml	Ask for price

Pex14 3'UTR GFP Stable Cell Line
TU166210	ABM	1.0 ml	Ask for price

Pex14 3'UTR Luciferase Stable Cell Line
TU216095	ABM	1.0 ml	Ask for price

Pex14 3'UTR GFP Stable Cell Line
TU266095	ABM	1.0 ml	Ask for price

PEX14 3'UTR GFP Stable Cell Line
TU067755	ABM	1.0 ml	EUR 2333

PEX14 Protein Vector (Rat) (pPB-C-His)
PV293450	ABM	500 ng	EUR 603

PEX14 Protein Vector (Rat) (pPB-N-His)
PV293451	ABM	500 ng	EUR 603

PEX14 Protein Vector (Rat) (pPM-C-HA)
PV293452	ABM	500 ng	EUR 603

PEX14 Protein Vector (Rat) (pPM-C-His)
PV293453	ABM	500 ng	EUR 603

PEX14 Protein Vector (Mouse) (pPB-C-His)
PV215182	ABM	500 ng	EUR 603

PEX14 Protein Vector (Mouse) (pPB-N-His)
PV215183	ABM	500 ng	EUR 603

PEX14 Protein Vector (Mouse) (pPM-C-HA)
PV215184	ABM	500 ng	EUR 603

PEX14 Protein Vector (Mouse) (pPM-C-His)
PV215185	ABM	500 ng	EUR 603

PEX14 Protein Vector (Human) (pPB-C-His)
PV030841	ABM	500 ng	EUR 329

PEX14 Protein Vector (Human) (pPB-N-His)
PV030842	ABM	500 ng	EUR 329

PEX14 Protein Vector (Human) (pPM-C-HA)
PV030843	ABM	500 ng	EUR 329

PEX14 Protein Vector (Human) (pPM-C-His)
PV030844	ABM	500 ng	EUR 329

Pex14 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4712302	ABM	1.0 ug DNA	EUR 154

Pex14 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4712303	ABM	1.0 ug DNA	EUR 154

Pex14 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4712304	ABM	1.0 ug DNA	EUR 154

Pex14 sgRNA CRISPR Lentivector (Rat) (Target 1)
K7067602	ABM	1.0 ug DNA	EUR 154

Pex14 sgRNA CRISPR Lentivector (Rat) (Target 2)
K7067603	ABM	1.0 ug DNA	EUR 154

Pex14 sgRNA CRISPR Lentivector (Rat) (Target 3)
K7067604	ABM	1.0 ug DNA	EUR 154

PEX14 sgRNA CRISPR Lentivector (Human) (Target 1)
K1629702	ABM	1.0 ug DNA	EUR 154

PEX14 sgRNA CRISPR Lentivector (Human) (Target 2)
K1629703	ABM	1.0 ug DNA	EUR 154

PEX14 sgRNA CRISPR Lentivector (Human) (Target 3)
K1629704	ABM	1.0 ug DNA	EUR 154

PEX14 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV693781	ABM	1.0 ug DNA	EUR 682

PEX14 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV693785	ABM	1.0 ug DNA	EUR 682

PEX14 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV693786	ABM	1.0 ug DNA	EUR 682

Human Peroxisomal Biogenesis Factor 14 (PEX14) ELISA Kit
abx382160-96tests	Abbexa	96 tests	EUR 911

Recombinant Pichia pastoris PEX14 Protein (aa 1-425)
VAng-Cr6650-1mgEcoli	Creative Biolabs	1 mg (E. coli)	EUR 4903
Description: Pichia pastoris Peroxisomal membrane protein PEX14 (PEX14), recombinant protein.

Recombinant Pichia pastoris PEX14 Protein (aa 1-425)
VAng-Cr6650-500gEcoli	Creative Biolabs	500 µg (E. coli)	EUR 3459
Description: Pichia pastoris Peroxisomal membrane protein PEX14 (PEX14), recombinant protein.

Recombinant Pichia pastoris PEX14 Protein (aa 1-425)
VAng-Cr6650-50gEcoli	Creative Biolabs	50 µg (E. coli)	EUR 2360
Description: Pichia pastoris Peroxisomal membrane protein PEX14 (PEX14), recombinant protein.

Recombinant Saccharomyces Cerevisiae PEX14 Protein (aa 2-341) [His]
VAng-Wyb5277-1mg	Creative Biolabs	1 mg	EUR 4565
Description: Saccharomyces Cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) Peroxisomal Biogenesis Factor 14, recombinant protein.

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4712305	ABM	3 x 1.0 ug	EUR 376

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7067605	ABM	3 x 1.0 ug	EUR 376

PEX14 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K1629705	ABM	3 x 1.0 ug	EUR 376

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4712306	ABM	1.0 ug DNA	EUR 167

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4712307	ABM	1.0 ug DNA	EUR 167

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K4712308	ABM	1.0 ug DNA	EUR 167

PEX14 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV693782	ABM	1.0 ug DNA	EUR 682

PEX14 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV693783	ABM	1.0 ug DNA	EUR 740

PEX14 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV693784	ABM	1.0 ug DNA	EUR 740

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K7067606	ABM	1.0 ug DNA	EUR 167

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K7067607	ABM	1.0 ug DNA	EUR 167

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K7067608	ABM	1.0 ug DNA	EUR 167

PEX14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K1629706	ABM	1.0 ug DNA	EUR 167

PEX14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K1629707	ABM	1.0 ug DNA	EUR 167

PEX14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K1629708	ABM	1.0 ug DNA	EUR 167

Together, these results suggest that Pex14p a unique factor that functions in the dual process in peroxisomal biogenesis and degradation with Pex5p coordination in response to environmental changes.