Identification and functional characterization of Trypanosoma brucei peroxin 16.

Protozoan parasites of the family Trypanosomatidae infect humans as well as livestock such devastating disease causing sleeping sickness, Chagas disease and Leishmaniasis. This parasite glycolytic enzymes in the organelle through a unique, glycosomes.

Glycosomes is a subclass of peroxisomes and they are essential to the survival of the parasite. Therefore, interference glycosome biogenesis is an attractive drug target for this Neglected Tropical Diseases (NTDs). Peroxin 16 (PEX16) plays an important role in peroxisomal membrane protein targeting and de novo biogenesis of the peroxisomes from the endoplasmic reticulum (ER).

We identify trypanosomal PEX16 based on characteristics of a particular sequence and indicates that it is an integral membrane protein glycosomal of procyclic and bloodstream trypanosomes form. RNAi-mediated knockdown partial PEX16 Trypanosoma brucei bloodstream form trypanosomes causing severe ATP depletion, disability motility and cell death. Microscopic and biochemical analyzes revealed a drastic reduction glycosome and mislocalization amount of enzyme matrix glycosomal to the cytosol. Glycosomes localization remaining asymmetry observed with severe depletion in the posterior part.

The results showed that PEX16 trypanosomal very important for the biogenesis glycosome and thus, providing a potential drug target for sleeping sickness and related diseases.

Peroxin Pex14p are key components for autophagic degradation of mammalian peroxisomes coordinated by directly binding LC3-II.

Pexophagy can be experimentally induced in mammalian cells by removing serum culture. Pex14p, an important membrane protein peroxisomal for imported protein matrix in docking receptor soluble Pex5p, is involved in the degradation of autophagic mammals from peroxisomes and interact with forms lipidated of LC3, called LC3-II, is an important factor for the formation of autophagosome, under conditions of starvation in cell CHO-K1. However, the molecular mechanisms underlying the interaction Pex14p-LC3-II is still unknown.

To verify whether Pex14p directly bind LC3-II, we reconstituted in vitro system of conjugation for the synthesis of LC3-II. We show here that Pex14p directly interact with LC3-II through the transmembrane domain of Pex14p. Pex5p competitively inhibits this interaction, implying that Pex14p preferential binding to Pex5p under rich conditions nutrisi-. In addition, mutants defective in the import Pex5p PTS1-protein loses its affinity for Pex14p under conditions of malnutrition, so it might explain why Pex14p preferentially interact with LC3-II under starvation conditions in vivo.

PEX14 antibody

70R-19219 50 ul
EUR 435
Description: Rabbit polyclonal PEX14 antibody

PEX14 Antibody

DF4284 200ul
EUR 304
Description: PEX14 Antibody detects endogenous levels of total PEX14.

PEX14 Antibody

1-CSB-PA017800ESR1HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

PEX14 Antibody

1-CSB-PA017800ESR2HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

PEX14 Antibody

1-CSB-PA017800GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IF

PEX14 Antibody

1-CSB-PA867033LA01DOA
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Arabidopsis thaliana. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:500-1:2000

PEX14 Antibody

1-CSB-PA003741
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/20000

PEX14 Antibody

CSB-PA775947-
EUR 335
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

PEX14 Antibody

CSB-PA775947-100ul 100ul
EUR 316
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100

PEX14 siRNA

20-abx928278
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PEX14 siRNA

20-abx928279
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PEX14 Antibody

34892-100ul 100ul
EUR 252

PEX14 Antibody

34892-50ul 50ul
EUR 187

PEX14 siRNA

20-abx903947
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

Human Peroxisomal membrane protein PEX14, PEX14 ELISA KIT

ELI-35662h 96 Tests
EUR 824

Mouse Peroxisomal membrane protein PEX14, Pex14 ELISA KIT

ELI-37738m 96 Tests
EUR 865

Anti-PEX14 (1G12)

YF-MA14671 100 ug
EUR 363
Description: Mouse monoclonal to PEX14

PEX14 Blocking Peptide

DF4284-BP 1mg
EUR 195

Anti-PEX14 antibody

PAab06327 100 ug
EUR 355

PEX14 cloning plasmid

CSB-CL017800HU-10ug 10ug
EUR 233
Description: A cloning plasmid for the PEX14 gene.

PEX14 Polyclonal Antibody

ES8932-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against PEX14 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

PEX14 Polyclonal Antibody

ES8932-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against PEX14 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

PEX14 Blocking Peptide

20-abx063068
  • EUR 272.00
  • EUR 411.00
  • 1 mg
  • 5 mg

Anti-PEX14 Antibody

A03327 100ul
EUR 397
Description: Rabbit Polyclonal PEX14 Antibody. Validated in IHC, WB and tested in Human, Mouse.

PEX14 Polyclonal Antibody

ABP59881-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of PEX14 from Human, Mouse, Rat. This PEX14 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX14 protein

PEX14 Polyclonal Antibody

ABP59881-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of PEX14 from Human, Mouse, Rat. This PEX14 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX14 protein

PEX14 Polyclonal Antibody

ABP59881-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of PEX14 from Human, Mouse, Rat. This PEX14 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human PEX14 protein

Anti-PEX14 antibody

STJ29475 100 µl
EUR 277
Description: This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.

Anti-PEX14 antibody

STJ99650 200 µl
EUR 197
Description: Rabbit polyclonal to PEX14.

PEX14 Rabbit pAb

A7336-100ul 100 ul
EUR 308

PEX14 Rabbit pAb

A7336-200ul 200 ul
EUR 459

PEX14 Rabbit pAb

A7336-20ul 20 ul
EUR 183

PEX14 Rabbit pAb

A7336-50ul 50 ul
EUR 223

PEX14 Conjugated Antibody

C34892 100ul
EUR 397

anti- PEX14 antibody

FNab06327 100µg
EUR 505.25
Description: Antibody raised against PEX14

PEX14 ELISA KIT|Human

EF001689 96 Tests
EUR 689

PEX14 Antibody, HRP conjugated

1-CSB-PA867033LB01DOA
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Arabidopsis thaliana. This antibody is HRP conjugated. Tested in the following application: ELISA

PEX14 Antibody, FITC conjugated

1-CSB-PA867033LC01DOA
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Arabidopsis thaliana. This antibody is FITC conjugated. Tested in the following application: ELISA

PEX14 Antibody, Biotin conjugated

1-CSB-PA867033LD01DOA
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against PEX14. Recognizes PEX14 from Arabidopsis thaliana. This antibody is Biotin conjugated. Tested in the following application: ELISA

PEX14 Recombinant Protein (Human)

RP023131 100 ug Ask for price

PEX14 Recombinant Protein (Mouse)

RP161384 100 ug Ask for price

PEX14 Recombinant Protein (Rat)

RP220085 100 ug Ask for price

Mouse PEX14 shRNA Plasmid

20-abx974669
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Rat PEX14 shRNA Plasmid

20-abx986246
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human PEX14 shRNA Plasmid

20-abx953467
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Polyclonal PEX14 Antibody (Center)

AMM07100G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PEX14 (Center). This antibody is tested and proven to work in the following applications:

Pex14 ORF Vector (Rat) (pORF)

ORF073363 1.0 ug DNA
EUR 506

PEX14 ORF Vector (Human) (pORF)

ORF007711 1.0 ug DNA
EUR 95

Pex14 ORF Vector (Mouse) (pORF)

ORF053796 1.0 ug DNA
EUR 506

Pex14 sgRNA CRISPR Lentivector set (Mouse)

K4712301 3 x 1.0 ug
EUR 339

Pex14 sgRNA CRISPR Lentivector set (Rat)

K7067601 3 x 1.0 ug
EUR 339

PEX14 sgRNA CRISPR Lentivector set (Human)

K1629701 3 x 1.0 ug
EUR 339

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

20-abx114405
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

abx034246-400ul 400 ul
EUR 523

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

abx034246-80l 80 µl
EUR 286

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

abx036238-100ug 100 ug
EUR 391

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

20-abx014702
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • EUR 70.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg
  • 5 ug

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

20-abx006919
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

20-abx008877
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

abx026888-400ul 400 ul
EUR 523

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

abx026888-80l 80 µl
EUR 286

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

20-abx320904
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

20-abx320905
  • EUR 439.00
  • EUR 328.00
  • 100 ul
  • 50 ul

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

abx236327-100ug 100 ug
EUR 481

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

abx331597-100ul 100 ul
EUR 425

Peroxisomal Biogenesis Factor 14 (PEX14) Antibody

20-abx328657
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

PEX14 3'UTR Luciferase Stable Cell Line

TU017755 1.0 ml
EUR 2333

Pex14 3'UTR Luciferase Stable Cell Line

TU116210 1.0 ml Ask for price

Pex14 3'UTR GFP Stable Cell Line

TU166210 1.0 ml Ask for price

Pex14 3'UTR Luciferase Stable Cell Line

TU216095 1.0 ml Ask for price

Pex14 3'UTR GFP Stable Cell Line

TU266095 1.0 ml Ask for price

PEX14 3'UTR GFP Stable Cell Line

TU067755 1.0 ml
EUR 2333

PEX14 Protein Vector (Rat) (pPB-C-His)

PV293450 500 ng
EUR 603

PEX14 Protein Vector (Rat) (pPB-N-His)

PV293451 500 ng
EUR 603

PEX14 Protein Vector (Rat) (pPM-C-HA)

PV293452 500 ng
EUR 603

PEX14 Protein Vector (Rat) (pPM-C-His)

PV293453 500 ng
EUR 603

PEX14 Protein Vector (Mouse) (pPB-C-His)

PV215182 500 ng
EUR 603

PEX14 Protein Vector (Mouse) (pPB-N-His)

PV215183 500 ng
EUR 603

PEX14 Protein Vector (Mouse) (pPM-C-HA)

PV215184 500 ng
EUR 603

PEX14 Protein Vector (Mouse) (pPM-C-His)

PV215185 500 ng
EUR 603

PEX14 Protein Vector (Human) (pPB-C-His)

PV030841 500 ng
EUR 329

PEX14 Protein Vector (Human) (pPB-N-His)

PV030842 500 ng
EUR 329

PEX14 Protein Vector (Human) (pPM-C-HA)

PV030843 500 ng
EUR 329

PEX14 Protein Vector (Human) (pPM-C-His)

PV030844 500 ng
EUR 329

Pex14 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4712302 1.0 ug DNA
EUR 154

Pex14 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4712303 1.0 ug DNA
EUR 154

Pex14 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4712304 1.0 ug DNA
EUR 154

Pex14 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7067602 1.0 ug DNA
EUR 154

Pex14 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7067603 1.0 ug DNA
EUR 154

Pex14 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7067604 1.0 ug DNA
EUR 154

PEX14 sgRNA CRISPR Lentivector (Human) (Target 1)

K1629702 1.0 ug DNA
EUR 154

PEX14 sgRNA CRISPR Lentivector (Human) (Target 2)

K1629703 1.0 ug DNA
EUR 154

PEX14 sgRNA CRISPR Lentivector (Human) (Target 3)

K1629704 1.0 ug DNA
EUR 154

PEX14 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV693781 1.0 ug DNA
EUR 682

PEX14 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV693785 1.0 ug DNA
EUR 682

PEX14 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV693786 1.0 ug DNA
EUR 682

Human Peroxisomal Biogenesis Factor 14 (PEX14) ELISA Kit

abx382160-96tests 96 tests
EUR 911

Recombinant Pichia pastoris PEX14 Protein (aa 1-425)

VAng-Cr6650-1mgEcoli 1 mg (E. coli)
EUR 4903
Description: Pichia pastoris Peroxisomal membrane protein PEX14 (PEX14), recombinant protein.

Recombinant Pichia pastoris PEX14 Protein (aa 1-425)

VAng-Cr6650-500gEcoli 500 µg (E. coli)
EUR 3459
Description: Pichia pastoris Peroxisomal membrane protein PEX14 (PEX14), recombinant protein.

Recombinant Pichia pastoris PEX14 Protein (aa 1-425)

VAng-Cr6650-50gEcoli 50 µg (E. coli)
EUR 2360
Description: Pichia pastoris Peroxisomal membrane protein PEX14 (PEX14), recombinant protein.

Recombinant Saccharomyces Cerevisiae PEX14 Protein (aa 2-341) [His]

VAng-Wyb5277-1mg 1 mg
EUR 4565
Description: Saccharomyces Cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) Peroxisomal Biogenesis Factor 14, recombinant protein.

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4712305 3 x 1.0 ug
EUR 376

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7067605 3 x 1.0 ug
EUR 376

PEX14 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1629705 3 x 1.0 ug
EUR 376

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4712306 1.0 ug DNA
EUR 167

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4712307 1.0 ug DNA
EUR 167

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4712308 1.0 ug DNA
EUR 167

PEX14 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV693782 1.0 ug DNA
EUR 682

PEX14 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV693783 1.0 ug DNA
EUR 740

PEX14 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV693784 1.0 ug DNA
EUR 740

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7067606 1.0 ug DNA
EUR 167

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7067607 1.0 ug DNA
EUR 167

Pex14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7067608 1.0 ug DNA
EUR 167

PEX14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1629706 1.0 ug DNA
EUR 167

PEX14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1629707 1.0 ug DNA
EUR 167

PEX14 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1629708 1.0 ug DNA
EUR 167

Together, these results suggest that Pex14p a unique factor that functions in the dual process in peroxisomal biogenesis and degradation with Pex5p coordination in response to environmental changes.

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